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一年来,许多全国人大代表和谭新萍一样,用脚步丈量民情,用心倾听民意。从社区网格到田野阡陌,从代表之家、代表联络站到基层立法联系点,代表联系群众的渠道日益拓宽,举措日益精准。他们用实干担当书写着履职答卷,诠释着“人民选我当代表,我当代表为人民”的庄严承诺。
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Funding limitations prevented Nyren from dedicating much time to the project at first, but in 1993, he was finally able to publish a proof-of-principle. His technique began by mixing the template DNA with a primer, a single dNTP, and three enzymes: the familiar DNA polymerase plus the light cascade enzymes, ATP sulfurylase and firefly luciferase.6 If the dNTP was incorporated into a strand of DNA, PPi would be produced in the chemical reaction. ATP sulfurylase could then convert the PPi into ATP, which would provide energy for the luciferase enzyme, producing light. Thus, it was possible to determine each base in the sequence by cycling through the dNTPs one at a time until light was detected, and then washing extra nucleotides out between each step. By literally rinsing and repeating, the sequence could be recorded one letter at a time without the use of any gels, which often took hours to run and were difficult to automate.,这一点在一键获取谷歌浏览器下载中也有详细论述
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